AI Article Synopsis
- This study compares saliva-based whole-genome sequencing (WGS) to blood-based WGS to assess accuracy and reliability.
- A novel technique called pseudo-replication was developed to better understand the discrepancies in genotype results between the two methods.
- The research also characterized individual salivary microbiomes using non-human DNA in saliva, finding that observed discordances mainly occurred in low-confidence genomic regions and aligning with expected results from technical replicates.
Article Abstract
This study sets out to establish the suitability of saliva-based whole-genome sequencing (WGS) through a comparison against blood-based WGS. To fully appraise the observed differences, we developed a novel technique of pseudo-replication. We also investigated the potential of characterizing individual salivary microbiomes from non-human DNA fragments found in saliva. We observed that the majority of discordant genotype calls between blood and saliva fell into known regions of the human genome that are typically sequenced with low confidence; and could be identified by quality control measures. Pseudo-replication demonstrated that the levels of discordance between blood- and saliva-derived WGS data were entirely similar to what one would expect between technical replicates if an individual's blood or saliva had been sequenced twice. Finally, we successfully sequenced salivary microbiomes in parallel to human genomes as demonstrated by a comparison against the Human Microbiome Project.
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| http://dx.doi.org/10.1002/gepi.22386 | DOI Listing |
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Article Synopsis
- Pregnancy involves significant hormonal and metabolic changes to support fetal development, which the study investigates through changes in the salivary microbiome and biochemical markers between the second and third trimesters.
- Saliva samples from 45 pregnant women were analyzed using advanced DNA sequencing techniques, revealing notable shifts in microbial diversity, including a 3-fold increase in Bacteroidota and changes in other microbial taxa.
- Biochemical changes included increased BMI, pulse rate, glucose, and cholesterol levels in the third trimester, along with correlations between these factors and microbial abundance, emphasizing the need for monitoring oral health and metabolic health during pregnancy.
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