We present a protocol for in vivo siRNA-mediated knockdown of a gene of interest in mouse liver using systemic delivery via intravenous injection. We describe a step-by-step protocol for delivery of siRNA particles, with tips on how to optimize dosage. We detail steps for feeding/starving cycles as well as for liver tissue isolation, followed by gene expression analysis, measured at the mRNA and protein levels. For complete information on the generation and use of this protocol, please refer to Wrobel et al. (2020).
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102171 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2021.100500 | DOI Listing |
Publication Analysis
Top Keywords
liver tissue
8
transient sirna-mediated
4
sirna-mediated protein
4
protein knockdown
4
knockdown mouse
4
mouse feeding/starving
4
feeding/starving cycle
4
cycle liver
4
tissue analysis
4
analysis protocol
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!