Digital holographic microscopy evaluation of dynamic cell response to electroporation.

Biomed Opt Express

Biophysics and Cellular Biotechnology Department, Faculty of Medicine, University of Medicine and Pharmacy Carol Davila, 8 Eroii Sanitari Blvd., Bucharest, 050474, Romania.

Published: April 2021

Phase-derived parameters and time autocorrelation functions were used to analyze the behavior of murine B16 cells exposed to different amplitudes of electroporation pulses. Cells were observed using an digital holographic microscope equipped with a fast camera. Series of quantitative phase images of cells were reconstructed and further processed using MATLAB codes. Projected area, dry mass density, and entropy proved to be predictors for permeabilized cells that swell or collapse. Autocorrelation functions of phase fluctuations in different regions of the cell showed a good correlation with the local effectiveness of permeabilization.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086444PMC
http://dx.doi.org/10.1364/BOE.421959DOI Listing

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