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A Python script to merge Sanger sequences. | LitMetric
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A Python script to merge Sanger sequences.

Cen Chen Bingguo Lu Xiaofang Huang Chuyun Bi Lili Zhao Yunzhuo Hu Xuanyang Chen Shiqiang Lin Kai Huang

PeerJ

Clinical Center for Human Genomic Research, Union Hospital, Huazhong University of Science and Technology, Wuhan, China.

Published: April 2021

Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to assemble multiple overlapping Sanger sequences. The script utilizes the overlapping regions within the tandem Sanger sequences to merge the Sanger sequences. The results demonstrate that the script can produce the merged sequence from the input Sanger sequences in a single run. The script offers a simple and free method for merging Sanger sequences and is useful for gene cloning.

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 Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086567PMC
http://dx.doi.org/10.7717/peerj.11354DOI Listing

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