AI Article Synopsis
- Transgenes, when stably integrated into plant genomes, can behave differently in terms of epigenetics compared to natural genes, especially regarding their susceptibility to DNA methylation.
- The study shows that the demethylase JMJ14 is responsible for mitigating DNA methylation of transgenes, while IBM1 specifically affects endogenous genes.
- This differential regulation by JMJ14 can lead to the creation of abnormal RNAs, which trigger post-transcriptional silencing of the transgenes, indicating that the plant's genome treats these transgenes as separate entities initially.
Article Abstract
Transgenes that are stably expressed in plant genomes over many generations could be assumed to behave epigenetically the same as endogenous genes. Here, we report that whereas the histone H3K9me2 demethylase IBM1, but not the histone H3K4me3 demethylase JMJ14, counteracts DNA methylation of Arabidopsis endogenous genes, JMJ14, but not IBM1, counteracts DNA methylation of expressed transgenes. Additionally, JMJ14-mediated specific attenuation of transgene DNA methylation enhances the production of aberrant RNAs that readily induce systemic post-transcriptional transgene silencing (PTGS). Thus, the JMJ14 chromatin modifying complex maintains expressed transgenes in a probationary state of susceptibility to PTGS, suggesting that the host plant genome does not immediately accept expressed transgenes as being epigenetically the same as endogenous genes.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119426 | PMC |
| http://dx.doi.org/10.1038/s41467-021-22995-3 | DOI Listing |
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