Highly simple, sensitive and selective method is developed for the spectrophotometric determination of Metronidazole (MDZ) antibacterial drug either in pure form or in pharmaceutical formulations. This method is based on reduction of potassium permanganate by Metronidazole drug in sodium hydroxide solution to give green manganate ion which recorded at 610 nm. The method produced linear responses in the concentration range 4.28 - 59.91 µg mL with limit of detection (LOD) and limit of quantification (LOQ) 0.21 and 0.69 µg mL for Metronidazole drug respectively. The apparent molar absorptivity is 0.865 × 10 L mol cm, Sandell sensitivity is 0.019 µg cm and correlation coefficient is 0.951. The method is highly reproducible and has been applied to a wide variety of pharmaceutical formulations and the results compare favourably with those of official methods.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.saa.2021.119858 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Preliminary Data on Metabolites: Comprehensive Characterization, Antioxidant, Antidiabetic, Antimicrobial Activities, LC-MS/MS Profiling, and Predicted ADMET Analysis.
Metabolites
January 2025
Univ Rennes, Ecole Nationale Supérieure de Chimie de Rennes, CNRS, ISCR-UMR6226, 35000 Rennes, France.
Background/objectives: extract, obtained via microwave-enhanced extraction, was evaluated for its antioxidant, antidiabetic, and antimicrobial activities to explore its therapeutic potential.
Methods: The extraction was performed using microwave-enhanced techniques, and LC-MS/MS was employed to profile the metabolites in the extract. Total phenolic and flavonoid contents were quantified using spectrophotometric methods.
Polydeoxynucleotide-Loaded Visible Light Photo-Crosslinked Gelatin Methacrylate Hydrogel: Approach to Accelerating Cartilage Regeneration.
Gels
January 2025
Department of Polymer Nano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of Korea.
Articular cartilage faces challenges in self-repair due to the lack of blood vessels and limited chondrocyte concentration. Polydeoxyribonucleotide (PDRN) shows promise for promoting chondrocyte growth and cartilage regeneration, but its delivery has been limited to injections. Continuous PDRN delivery is crucial for effective cartilage regeneration.
View Article and Find Full Text PDFAnti-arthritic Effects of Undifferentiated and Chondrogenic Differentiated MSCs in MIA-induced Osteoarthritis in Wistar Rats: Involvement of Oxidative Stress and Immune Modulation.
Curr Stem Cell Res Ther
January 2025
Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, P.O. Box 62521, Beni-Suef, Egypt.
Introduction: Osteoarthritis (OA) is a degenerative joint disease that can affect the many tissues of the joint. There are no officially recognized disease-modifying therapies for clinical use at this time probably due to a lack of complete comprehension of the pathogenesis of the disease. In recent years, emerging regenerative therapy and treatments with stem cells both undifferentiated and differentiated cells have gained much attention as they can efficiently promote tissue repair and regeneration.
View Article and Find Full Text PDFA Cost-Effective Nonaqueous Reversed-Phase High-Performance Liquid Chromatography Method to Measure Vitamin D3 in Hen's Egg Yolk.
J Sep Sci
January 2025
Department of Pharmaceutical Sciences, University of Perugia, Perugia, Italy.
The objective of this study is to develop an HPLC-UV method for the cost-effective and quantitative determination of vitamin D3 in food, even in the presence of vitamin D2, with a specific focus on egg yolk. During method development, the performance of three stationary phases in resolving the peak of vitamin D2 from that of vitamin D3 was investigated. The physicochemical properties of these phases differed particularly in the extent of hydrophobicity and silanophilic activity, including a GraceSmart RP C18 column without silanol endcapping, a Robusta RP C18 column with silanol endcapping, and a Waters Xbridge RP C18 column with ethylene-bridged hybrid (BEH) particle technology.
View Article and Find Full Text PDFDevelopment and validation of a UV-Vis spectrophotometric method for estimation of total content of chalcone.
MethodsX
June 2025
Observatory of Drug-Herb Interactions, Faculty of Pharmacy, University of Coimbra, Health Science Campus, Azinhaga Santa Comba, Coimbra, Portugal.
In this study, a straightforward spectrophotometric method was developed for quantifying the total content of chalcones in a sample. The method exhibits linearity, accuracy, precision, repeatability, and enables the estimation of total chalcone content in trans-chalcone equivalents for a sample diluted in carbon tetrachloride and added to antimony pentachloride. The analytical wavelength was determined to be 390 nm.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!