AI Article Synopsis
- * It emphasizes the importance of optimizing gene expression and regulation to balance growth and production in these factories, focusing on three regulation stages: genomic, mRNA, and protein.
- * The review highlights current technologies and genetic-engineering tools used in constructing microbial cell factories, along with their applications, while also exploring future strategies for improving gene expression and regulation.
Article Abstract
To overcome environmental problems caused by the use of fossil resources, microbial cell factories have become a promising technique for the sustainable and eco-friendly development of valuable products from renewable resources. Constructing microbial cell factories with high titers, yields, and productivity requires a balance between growth and production; to this end, tuning gene expression and regulation is necessary to optimise and precisely control complicated metabolic fluxes. In this article, we review the current trends and advances in tuning gene expression and regulation and consider their engineering at each of the three stages of gene regulation: genomic, mRNA, and protein. In particular, the technological approaches utilised in a diverse range of genetic-engineering-based tools for the construction of microbial cell factories are reviewed and representative applications of these strategies are presented. Finally, the prospects for strategies and systems for tuning gene expression and regulation are discussed.
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| http://dx.doi.org/10.1016/j.biotechadv.2021.107767 | DOI Listing |
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