AI Article Synopsis
- Shugoshin proteins play a crucial role in protecting centromeric cohesin during both mitosis and meiosis.
- The study focuses on the interaction between human hSgo1 and the PP2A-B56 phosphatase, uncovering new details about their structural formation and functional dynamics.
- Key findings show that a specific conserved pocket on B56 is essential for hSgo1 binding, which inhibits the binding of certain substrates and allows for the dephosphorylation of Cdk1 sites on hSgo1, thereby influencing cohesin interactions.
Article Abstract
The shugoshin proteins are universal protectors of centromeric cohesin during mitosis and meiosis. The binding of human hSgo1 to the PP2A-B56 phosphatase through a coiled-coil (CC) region mediates cohesion protection during mitosis. Here we undertook a structure function analysis of the PP2A-B56-hSgo1 complex, revealing unanticipated aspects of complex formation and function. We establish that a highly conserved pocket on the B56 regulatory subunit is required for hSgo1 binding and cohesion protection during mitosis in human somatic cells. Consistent with this, we show that hSgo1 blocks the binding of PP2A-B56 substrates containing a canonical B56 binding motif. We find that PP2A-B56 bound to hSgo1 dephosphorylates Cdk1 sites on hSgo1 itself to modulate cohesin interactions. Collectively our work provides important insight into cohesion protection during mitosis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256288 | PMC |
| http://dx.doi.org/10.15252/embr.202052295 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Equalization of basic public services enabled by digitization: A study of mechanism and heterogeneity.
PLoS One
January 2025
School of Finance, Lanzhou University of Finance and Economics, Lanzhou, PR China.
Digitalization has penetrated into every aspect of life. However, research on the mechanisms through which digitalization affects the equalization of basic public services, as well as the heterogeneity of its impact on different fields of these services, is still insufficient. Based on the panel data of 30 provincial-level administrative regions in China from 2013 to 2023, this paper studies the mechanism and heterogeneity of the impact of digital development on the equalization of basic public services.
View Article and Find Full Text PDFUnveiling the Texture Secrets of Germplasm: Advanced Grading and Quality Assessment Through Texture Profile Analysis (TPA).
Foods
January 2025
Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China.
is an edible mushroom with medical applciations. To explore the correlation between the texture indices of and to establish a sensory quality evaluation system, the texture quality characteristics of 214 germplasm resources from our country were analyzed via the Texture Profile Analysis (TPA) method on a texture analyzer. The research revealed significant differences in the texture quality characteristics of both the pileus and stipe among populations.
View Article and Find Full Text PDFStructural and Physicochemical Properties of Glycerol-Plasticized Edible Films Made from Pea Protein-Based Emulsions Containing Increasing Concentrations of Candelilla Wax or Oleic Acid.
Molecules
December 2024
Department of Inorganic Chemistry, Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie-Sklodowska University in Lublin, Maria Curie-Sklodowska Sq. 3, 20-031 Lublin, Poland.
Hydrophobization could improve the moisture resistance of biopolymer-based materials, depending on the methods and materials used, providing benefits for packaging applications. The aim of this study was to compare the effect of increasing concentrations (0-2.0%) of candelilla wax (CW) and oleic acid (OA) on the structural and physicochemical properties, including water affinity, of glycerol-plasticized pea protein isolate (PPI) films.
View Article and Find Full Text PDFPreparation and Performance Study of Novel Foam Vegetation Concrete.
Materials (Basel)
December 2024
State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, Chengdu University of Technology, Chengdu 610059, China.
Vegetation concrete is one of the most widely used substrates in ecological slope protection, but its practical application often limits the growth and nutrient uptake of plant roots due to consolidation problems, which affects the effectiveness of slope protection. This paper proposed the use of a plant protein foaming agent as a porous modifier to create a porous, lightweight treatment for vegetation concrete. Physical performance tests, direct shear tests, plant growth tests, and scanning electron microscopy experiments were conducted to compare and analyze the physical, mechanical, microscopic characteristics, and phyto-capabilities of differently treated vegetation concrete.
View Article and Find Full Text PDFInfluence of Floral Strip Width on Spider and Carabid Beetle Communities in Maize Fields.
Insects
December 2024
Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, No. 31, Fukang Road, Nankai District, Tianjin 300191, China.
The study explored the impact of floral strip width on the spider and carabid beetle communities in maize fields over two years. Three widths of floral strips (2 m, 4 m, and 6 m) were compared with maize-only control strips to evaluate species diversity and distribution. The results showed significant differences in both spider and carabid populations between floral and control strips, with 4 m and 6 m widths consistently harboring higher biodiversity.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!