AI Article Synopsis
- Nannochloropsis oceanica is a promising microalga for producing valuable lipids and biofuels, but its current lipid yields are not economically viable.
- Researchers conducted a study to uncover genes linked to higher neutral lipid content by creating a library of mutants and performing cell sorting.
- A notable mutant (HLM23) exhibited a 40% increase in lipid content, improved photosynthesis, and no negative growth effects, revealing potential pathways for enhancing lipid production through genetic engineering.
Article Abstract
The microalga Nannochloropsis oceanica is considered a promising platform for the sustainable production of high-value lipids and biofuel feedstocks. However, current lipid yields of N. oceanica are too low for economic feasibility. Gaining fundamental insights into the lipid metabolism of N. oceanica could open up various possibilities for the optimization of this species through genetic engineering. Therefore, the aim of this study was to discover novel genes associated with an elevated neutral lipid content. We constructed an insertional mutagenesis library of N. oceanica, selected high lipid mutants by five rounds of fluorescence-activated cell sorting, and identified disrupted genes using a novel implementation of a rapid genotyping procedure. One particularly promising mutant (HLM23) was disrupted in a putative APETALA2-like transcription factor gene. HLM23 showed a 40%-increased neutral lipid content, increased photosynthetic performance, and no growth impairment. Furthermore, transcriptome analysis revealed an upregulation of genes related to plastidial fatty acid biosynthesis, glycolysis and the Calvin-Benson-Bassham cycle in HLM23. Insights gained in this work can be used in future genetic engineering strategies for increased lipid productivity of Nannochloropsis.
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| http://dx.doi.org/10.1016/j.ymben.2021.04.012 | DOI Listing |
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