Long noncoding RNAs act essential regulators in lung cancer tumorigenesis. Our research aimed to investigate the potential function and molecular mechanisms of MLK7-AS1 in NSCLC (non-small-cell lung cancer). QRT-PCR results indicated that the MLK7-AS1 expression level was upregulated in NSCLC cells and tissues. MLK7-AS1 strengthened cell migration and invasion in H1299 and A549 cells. Luciferase reporter assay found that MLK7-AS1 functioned as an endogenous sponge for miR-375-3p. Transwell assay results showed that miR-375-3p suppressed cell migration and invasion in H1299 and A549 cells. YWHAZ was confirmed as a target gene of miR-375-3p by Targetscan. YWHAZ overexpression promoted the invasion of H1299 and A549 cells. MLK7-AS1 upregulated YWHAZ expression and enhanced H1299 and A549 cell invasion by sponging miR-375-3p. MLK7-AS1 improved the metastasis ability of A549 . In conclusion, MLK7-AS1 was identified as a novel oncogenic RNA in NSCLC and can function as a potential therapeutic target for NSCLC treatment.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100187 | PMC |
| http://dx.doi.org/10.3389/fonc.2021.626036 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
MDM2 up-regulates the energy metabolism in NSCLC in a p53-independent manner.
Biochem Biophys Res Commun
January 2025
Institute of Cytology, Russian Academy of Sciences, 194064, St. Petersburg, Russia. Electronic address:
Although an E3 ligase MDM2 is the major negative regulator of the p53 tumor suppressor, a growing body of evidence suggests its p53-independent oncogenic properties. In particular, MDM2 has been shown to regulate serine metabolism independently of p53 status in several types of neoplasia, including NSCLC. Using the GSEA approach and publicly available molecular data on NSCLC tumors, our bioinformatics data suggest that MDM2 affects a number of metabolic genes, particularly those encoding components of the electron transport chain (ETC).
View Article and Find Full Text PDF[Non-small cell lung cancer-derived exosomal circular RNA circEZH2 activates fibroblasts by regulating the miR-495-3p / TPD52 axis and NF-κB pathway].
Zhonghua Zhong Liu Za Zhi
December 2024
Department of Respiratory and Critical Care Medicine, the Affiliated People's Hospital of Ningbo University, Ningbo315000, China.
To study the effects and mechanisms of activation of human lung fibroblasts (MRC-5) by exosomal RNA hsa _ circ _ 0006357 (circEZH2) derived from non-small cell lung cancer. Western blot was used to detect exosome molecular markers, reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) and cell invasion assays to detect the effect of non-small cell lung cancer-derived exosomes on MRC-5 activation. A circRNA microarray analysis was performed in serum exosomes from patients with non-small cell lung cancer (collected at Ningbo University People's Hospital, September 2023), and levels of circEZH2 were measured in serum exosomes from non-small cell lung cancer by RT-qPCR analysis.
View Article and Find Full Text PDFThe impact of POSTN on tumor cell behavior and the tumor microenvironment in lung adenocarcinoma.
Int Immunopharmacol
January 2025
Department of Shandong Provincial Key Laboratory of Precision Oncology, Shandong Cancer Hospital Affiliated to Shandong First Medical University, Shandong Academy of Medical Science, Jinan, Shandong, China; Department of Radiation Oncology, Shandong University Cancer Center, Jinan, Shandong, China. Electronic address:
Background: The role of cancer-associated fibroblasts (CAFs) in modulating the anti-tumor immune response in lung adenocarcinoma (LUAD) remains elusive, primarily due to the heterogeneous nature of these cells. This heterogeneity muddles the understanding of their impact on immunotherapy effectiveness.
Methods: We utilized the LUAD single-cell dataset to precisely classify tumor cells and CAFs.
Fluorofenidone enhances cisplatin efficacy in non-small cell lung cancer: a novel approach to inhibiting cancer progression.
Transl Lung Cancer Res
November 2024
Department of Oncology, The Second Xiangya Hospital, Central South University, Changsha, China.
Background: Non-small cell lung cancer (NSCLC), the most prevalent lung cancer subtype, presents significant treatment challenges. Cisplatin (CP)-based regimens are central to the treatment of multiple solid tumors, but its use is restricted due to its dose-related renal toxicity. We previously found that fluorofenidone {1-[3-fluorophenyl]-5-methyl-2-[(1H)]-pyridone (AKF-PD)} effectively reverses CP-induced acute kidney injury (AKI).
View Article and Find Full Text PDF[GINS1 Enhances Glycolysis, Proliferation and Metastasis in Lung Adenocarcinoma Cells by Activating the Notch/PI3K/AKT/mTORC1 Signaling Pathway].
Zhongguo Fei Ai Za Zhi
October 2024
Medical Laboratory Center, the Affiliated Cancer Hospital of Xinjiang Medical University, Urumqi 830011, China.
Article Synopsis
- * Researchers analyzed GINS1 expression in LUAD tissues compared to healthy controls using various techniques, including bioinformatics, immunohistochemistry, and qRT-PCR, and manipulated GINS1 levels in cancer cell lines to assess its effects on cell proliferation, migration, and invasion.
- * Initial results indicate variations in GINS1 expression between LUAD patients and healthy controls, and ongoing experiments aim to uncover the underlying molecular mechanisms, potentially identifying new therapeutic targets for LUAD treatment
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!