In the present investigation, hyperparasitic interaction between TM (MH590235) and was studied through scanning electron microscopy and chromatographic techniques. Dose-mortality responses showed an increase in mortality of larva with an increase in spore concentration. The LC value for isolate TM (MH590235) was 2.4 × 10 spores mL. The ditrophic interaction between and after 24 h revealed the adherence of conidia on stemmata, sensory setae, maxillary palpi and legs. After 48 h post-infection (hpi), germination of the conidia and appressorium formation was observed. Formation of hyphae and initiation of fungal networking was observed at 72 hpi. Complete ramification by mycelia and conidiogenesis of was observed over the mycosed cadaver after 168 hpi. Subsequently, typical sympodial conidiophores of bearing secondary spores were also observed. The metabolome profile of healthy larvae of revealed the presence of non-volatile organic compounds (NVOCs) like docosene, nonadecene, palmitic acid and heneicosane. However, the NVOC profile was different in the larvae hyperparasitized by The metabolites present in the infected cadaver were phthalate esters, hydroxyquebrachamine and lactones.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053635 | PMC |
http://dx.doi.org/10.1007/s13205-021-02760-5 | DOI Listing |
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