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Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile)-Ha antimicrobial peptide application as a feed additive. | LitMetric

AI Article Synopsis

  • - Microencapsulation enhances the stability of antimicrobial peptides (AMPs) like Ctx(Ile)-Ha, reducing toxicity and providing a new method for combating multidrug-resistant (MDR) bacteria.
  • - The study shows that Ctx(Ile)-Ha demonstrates strong antibacterial activity against several MDR bacteria, including various strains of Salmonella and Staphylococcus, while maintaining stability when microencapsulated.
  • - The microencapsulated peptide, made using specific polymers, significantly reduces hemolysis and targets the intestinal release of the antimicrobial, making it a promising oral feed additive for controlling infections in monogastric animals.

Article Abstract

Microencapsulation is a potential biotechnological tool, which can overcome antimicrobial peptides (AMP) instabilities and reduce toxic side effects. Thus, this study evaluates the antibacterial activities of the Ctx(Ile)-Ha AMP against multidrug-resistant (MDR) and non-resistant bacteria and develop and characterize peptide-loaded microparticles coated with the enteric polymers hydroxypropylmethylcellulose acetate succinate (HPMCAS) and hydroxypropylmethylcellulose phthalate (HPMCP). Ctx(Ile)-Ha was obtained by solid phase peptide synthesis (SPPS) method, purified and characterized by HPLC and Mass Spectrometry. The peptide exhibited potent antibiotic activities against Salmonella enteritidis, Salmonella typhimurium, Pseudomonas aeruginosa (MDR), Acinetobacter baumannii (MDR), and Staphylococcus aureus (MDR). Ctx(Ile)-Ha microencapsulation was performed by ionic gelation with high efficiency, maintaining the physical-chemical stability. Ctx(Ile)-Ha coated-microparticles were characterized by DSC, TGA, FTIR-Raman, XRD and SEM. Hemolytic activity assay demonstrated that hemolysis was decreased up to 95% compared to single molecule. In addition, in vitro release control profile simulating different portions of gastrointestinal tract was performed and showed the microcapsules' ability to protect the peptide and release it in the intestine, aiming pathogen's location, mainly by Salmonella sp. Therefore, use of microencapsulated Ctx(Ile)-Ha can be allowed as an antimicrobial controller in monogastric animal production as an oral feed additive (antimicrobial controller), being a valuable option for molecules with low therapeutic indexes or high hemolytic rates.

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Source
http://dx.doi.org/10.1016/j.ijbiomac.2021.05.011DOI Listing

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