NORMALIZING EFFECT OF ELECTROMAGNETIC STIMULATION ON BLOOD QUANTITATIVE INDICES IN DEPRESSED RATS ON THE BACKGROUND OF OXYTOCIN.

Depression is linked to systemic inflammation. There is an association between blood cell count and/or hematological inflammatory markers with depression symptoms. Oxytocin reduces the activity of brain regions that produce anxiety and might involve in the rehabilitation of depressive-like behavior. Repetitive EMS is used in the treatment of moderate depression. So, the goal of this investigation was to study the quantitative characteristics of blood cells after EMS on the background of oxytocin premedication of in depressed rats. In the implementation of the project the depressed rats (250-450 g) were used (n=20). For each task two groups of the animal were conducted: experimental group (with EMS) and control group (without EMS). For repetitive (10-days) EMF exposure, the following parameters were used: 10000 -15000 Hz frequency, 1,5 m/Tesla, for 15 min. An animal model of depression was received by subcutaneous injection of Clomipramine from 8 to 21 days of neonatal development. The blood counts were performed 2 months later after clomipramine injection using blood HumaCount 30 TS. This analyzer allows quantifying the number of red blood cells, white blood cells (agranulocytes and granulocytes), platelets, hemoglobin, the hematocrit in 1 ml blood of rats. The hematological analysis was performed 2 weeks later after oxytocin (4 mcg/per animal during 10 days) injection and EMS. The obtained results were processed using an adequate statistical program. In clomipramine -induced depressed rats, EMS independently or with simultaneous injection of Oxytocin caused normalization of blood cells count (Red blood cells, white blood cells and Platelets). More important was the simultaneous impact of EMS and oxytocin. In depressed rats, the oxytocin alone, or together with EMS can restore the blood cell imbalance.