AI Article Synopsis
- Scientists created a new type of nanozyme called rGO-GP, which is made by mixing a special substance from mushrooms with reduced graphene oxide.
- This new nanozyme works better than others at speeding up important chemical reactions, thanks to how it spreads in liquids.
- They found a way to use rGO-GP to detect a substance called L-cysteine in very small amounts, which could help in medical tests.
Article Abstract
A facile and green approach to the preparation of peroxidase-like nanozymes by reducing and functionalizing graphene oxide (rGO) with Ganoderma polysaccharide (GP) has been achieved in this work. Our results showed that the as-fabricated nanozyme, namely rGO-GP, possessed the excellent property of simulating peroxidase with higher catalytic activity compared with GO or rGO obtained by using chitosan, which may be due to the better dispersion of rGO-GP in the solution. Steady-state kinetics studies further showed that the catalytic process conformed to Michaelis-Menten equation and ping-pong mechanism. Benefiting from the excellent peroxidase property of rGO-GP, we have also successfully established a highly sensitive and selective colorimetric detection approach to trace detection of L-cysteine (L-Cys). The limit of detection (LOD) of L-cysteine is 0.1 μM and the linear detection range is 2-30 μM. Furthermore, the nanozyme was successfully applied for detecting L-cysteine in serum. This work therefore demonstrates the advantages of rGO-GP as an effective nanozyme in both its green synthesis and detecting application.
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| http://dx.doi.org/10.1007/s00216-021-03352-1 | DOI Listing |
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