Antigenotoxic potential of the fermentation broth produced by RNC-D .

Evaluate the chemopreventive potential of the extract from RNC-D. Concentrations of RNC-D extract were tested in HepG2/C3A cells to assess their genotoxic (comet assay), mutagenic (micronucleus test) and antigenotoxic potential (comet assay) . 400 and 40 μg/ml concentrations induced DNA lesions, whereas the 4 μg/ml induced a desmutagenic effect. Complementary tests indicated that the extract minimized the formation of reactive oxygen species induced by methyl methanesulfonate and normalized the loss of membrane potential. The quantification of cytokines indicated that TNF-α was immunostimulated by the extract. However, when administered in conjunction with the methyl methanesulfonate, the extract blocked the TNF-α release. The fermentation broth from RNC-D showed an antigenotoxic effect, and thus the potential to be used as chemopreventive compound.