AI Article Synopsis
- The study aims to address the lack of a suitable vaccine for Necrotic Enteritis (NE) in the Indian broiler industry by investigating the prevalence and characteristics of C. perfringens type G in affected chickens.
- Researchers screened intestinal scrapings and faecal contents, finding that over 51% of suspected cases contained the pathogenic C. perfringens type G, with some strains showing multidrug resistance.
- The prepared sonicated supernatant (SS) vaccine demonstrated a significantly better immunogenic response compared to traditional preparations, highlighting the importance of the TpeL toxin in NE and suggesting SS as an effective immunization method.
Article Abstract
Objective: Keeping in view, the constraints faced by the Indian broiler industry with lack of a suitable vaccine against Necrotic Enteritis (NE), a study has been proposed to explore the prevalence and detail characterization of C. perfringens type G in NE suspected broiler chicken in the process of suitable vaccine development.
Methods: Intestinal scrapings/faecal contents of NE suspected broiler chickens were screened to establish the prevalence of C.perfringens type G in broiler birds. A most pathogenic, highly resistant type G isolate of C. perfringens, bearing both tpeL and gapC gene was selected for preparation of three different vaccine formulations, and to evaluate their immunogenic potential in broiler birds.
Results: Screening of clinical samples of NE suspected broiler birds revealed C. perfringens type G, bearing gapC gene in 51.22% samples, of which 47.62% revealed tpeL gene. Seven of the tpeL type G isolates were comparatively more pathogenic for mice, of which, one exhibited multidrug resistance towards ciprofloxacin, norfloxacin, tetracycline and levofloxacin. The sonicated supernatant (SS) prepared from the selected tpeL and gapC positive isolate could maintain a significantly higher protective IgG response than toxoid and bacterin preparation from the 21st to 28day of age in immunized birds.
Conclusion: The additional TpeL toxin in C. perfringens type G has been proved to be an additional key biological factor in the pathogenesis of NE in broiler chickens. Considering the release of more immunogenic proteins, the SS proved to be a better immunogenic preparation against NE with a multiple immunization dose.
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| http://dx.doi.org/10.1016/j.anaerobe.2021.102377 | DOI Listing |
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