A methodology is described that allows for localized Ca release by photoexcitation. For this, cells are loaded with polymer capsules with integrated plasmonic nanoparticles, which reside in endo-lysosomes. The micrometer-sized capsules can be individually excited by near-infrared light from a light pointer, causing photothermal heating, upon which there is a rise in the free cytosolic Ca concentration ([Ca ] ). The [Ca ] can be analyzed with a Ca indicator fluorophore. In this way, it is possible to excite local lysosomal Ca release in a desired target cell.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11469046 | PMC |
| http://dx.doi.org/10.1002/adma.202008261 | DOI Listing |
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