State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, P.R. China.
Published: July 2022
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Article Abstract
Three new andrastin derivatives, 10-formyl andrastone A (), 10-demethylated andrastone A () and andrastin G (), together with four known andrastin analogues (-) were isolated from an endophytic fungus . Their structures were determined by 1 D, 2 D NMR, and the absolute configurations were further determined by experimental and calculated ECD spectra. Compound exhibited significant antibacterial activity against B with an MIC value of 6.25 g·mL. Compounds and showed remarkable inhibitory activities against with the MIC value of 6.25 g·mL, respectively.
Fungi are known to produce many chemically diversified metabolites, yet their ecological roles are not always fully understood. The blue cheese fungus Penicillium roqueforti thrives in different ecological niches and is known to produce a wide range of metabolites, including mycotoxins. Three P.
Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Pharmaceutical Sciences, Hainan University, Haikou, 570228, PR China. Electronic address:
The genus Penicillium has provided us with the household antibiotic penicillin and the well-known lipid-lowering agent mevastatin. The strain Penicillium sp. SZ-1 was found to grow vigorously in an intact Pinus koraiensis seed, it is inferred that the strain may develop unique mechanisms associated with the biosynthesis of rare metabolites.
Pyr4-family terpene cyclases are noncanonical transmembrane class II terpene cyclases that catalyze a variety of cyclization reactions in the biosynthesis of microbial terpenoids, such as meroterpenoids. However, although these cyclases are widely distributed in microorganisms, their three-dimensional structures have not been determined, possibly due to the transmembrane locations of these enzymes. In this chapter, we describe procedures for the functional analysis of transmembrane terpene cyclases based on their model structures generated using AlphaFold2.
A new andrastin-type meroterpenoid penimerodione A (), and three known analogues (-), were isolated from the culture of a marine-derived fungus HNNU w0032 by the guidance of MS/MS-based molecular networking. The planar structure of was established by extensive NMR spectroscopic and HRESIMS analyses, and the absolute configuration was elucidated by a single-crystal X-ray diffraction. Compound showed significant inhibitory effect on NO production in LPS-stimulated BV-2 macrophages with an IC value of 5.
The regulation of fungal specialized metabolism is a complex process involving various regulators. Among these regulators, LaeA, a methyltransferase protein originally discovered in spp., plays a crucial role.
