Highly Selective Detection of K Based on a Dimerized G-Quadruplex DNAzyme.

Anal Chem

State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, Liaoning 116023, China.

Published: May 2021

Potassium ion (K) plays a crucial role in biological systems, such as maintaining cellular processes and causing diseases. However, specifically, the detection of K is extremely challenging because of the coexistence of the chemically similar ion of Na under physiological conditions. In this work, a K specific biosensor is constructed on the basis of a dimerized G-quadruplex (GQ) DNA, which is promoted by K, and the enzymatic activity of the resulting DNAzyme depends on the concentration of the K. The K in a 1-200 mM concentration range can be selectively detected by visual color, UV-Vis absorbance or fluorescence even if the concentration of the accompanying Na is up to 140 mM at an ambient condition up to 45 °C. In addition, this system can also be used to selectively detect NH in a 5-200 mM concentration range. This dimerized DNAzyme offers a new type of biosensor with a potential application in the biological system.

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Source
http://dx.doi.org/10.1021/acs.analchem.1c00872DOI Listing

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