as a promising producer of recombinant hirudin.

The production of recombinant proteins in transgenic plants is becoming an increasingly serious alternative to classical biopharming methods as knowledge about this process grows. , an aquatic plant unique in its anatomy, is a promising expression system that can grow in submerged culture in bioreactors. In our study 8550 explants were subjected to -mediated transformation, and 41 independent hygromycin-resistant lines were obtained, with the transformation efficiency of 0.48%. 40 of them contained the gene (codon-optimized for expression in plants) and were independent lines of nuclear-transformed , the transgenic insertion has been confirmed by PCR and Southern blot analysis. We have analyzed the accumulation of the target protein and its expression has been proven in three transgenic lines. The maximum accumulation of recombinant hirudin was 0.02% of the total soluble protein, which corresponds to 775.5 ± 111.9 ng g of fresh weight of the plant. The results will be used in research on the development of an expression system based on plants for the production of hirudin and other recombinant pharmaceutical proteins.