A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 176

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 1034
Function: getPubMedXML

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3152
Function: GetPubMedArticleOutput_2016

File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

Electroporation-mediated transfection of Acholeplasma laidlawii with mycoplasma virus L1 and L3 DNA. | LitMetric
Browse Categories

Electroporation-mediated transfection of Acholeplasma laidlawii with mycoplasma virus L1 and L3 DNA.

A Lorenz W Just M da Silva Cardoso G Klotz

J Virol

Abteilung Virologie, Institut für Mikrobiologie, Universität Ulm, Federal Republic of Germany.

Published: August 1988

In contrast to mycoplasma virus L1 and L2 circular DNA, mycoplasma virus L3 linear DNA is not biologically active in polyethylene glycol-mediated transfection. Electroporation of Acholeplasma laidlawii, however, leads to plaque formation after incubation with L3 DNA. The efficiency of electroporation-mediated transfection is 1/10 that of polyethylene glycol-mediated transfection as estimated with L1 DNA. Trypsin treatment of cells before DNA addition increases the efficiency of DNA uptake.

Download full-text PDF

 Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC253748PMC
http://dx.doi.org/10.1128/JVI.62.8.3050-3052.1988DOI Listing

Publication Analysis

Top Keywords

mycoplasma virus
12
electroporation-mediated transfection
8
acholeplasma laidlawii
8
polyethylene glycol-mediated
8
glycol-mediated transfection
8
dna
7
transfection acholeplasma
4
laidlawii mycoplasma
4
virus dna
4
dna contrast
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!

Privacy Policy Site Map

© LitMetric 2025. All rights reserved.