AI Article Synopsis
- Researchers have used the SunTag method to visualize and measure the translation of single mRNAs into proteins in living embryos.
- The study specifically focuses on the mRNA translation of the protein Twist, which is important for cell development, revealing differences in translation efficiency across different regions.
- The findings highlight the presence of "translation factories" where clustered mRNAs are co-translated, providing insights into how gene regulation occurs during organism development.
Article Abstract
Much is known about the factors involved in the translation of messenger RNA (mRNA) into protein; however, this multistep process has not been imaged in living multicellular organisms. Here, we deploy the SunTag method to visualize and quantify the timing, location, and kinetics of the translation of single mRNAs in living embryos. By focusing on the translation of the conserved major epithelial-mesenchymal transition-inducing transcription factor Twist, we identify spatial heterogeneity in mRNA translation efficiency and reveal the existence of translation factories, where clustered mRNAs are cotranslated preferentially at basal perinuclear regions. Observing the location and dynamics of mRNA translation in a living multicellular organism opens avenues for understanding gene regulation during development.
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| http://dx.doi.org/10.1126/science.abc3483 | DOI Listing |
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