AI Article Synopsis
- The filamentous fungus is important for producing enzymes used in biotechnology and has a complex system for breaking down biomass, but more research is needed on how its genes are regulated.
- A null mutant strain was created to study the effects of deleting the xyloglucanase gene, revealing that it impacts the expression of cellulases and hemicellulases during sugarcane bagasse cultivation.
- The findings suggest that the CEL74A enzyme plays a key role in regulating these genes and that calcium may enhance the fungus's ability to break down xyloglucan, improving our understanding of fungal carbon utilization.
Article Abstract
Background: The filamentous fungus is used on an industrial scale to produce enzymes of biotechnological interest. This fungus has a complex cellulolytic system involved in the degradation of lignocellulosic biomass. However, several aspects related to the regulation of the expression of holocellulolytic genes and the production of cellulases by this fungus are still understood.
Methods: Here, we constructed a null mutant strain for the xyloglucanase gene and performed the characterization of the Δ strain to evaluate the genetic regulation of the holocellulases during sugarcane bagasse (SCB) cultivation.
Results: Our results demonstrate that the deletion of xyloglucanase may impact the regulation of holocellulase expression during SCB cultivation. The expression of cellulases , , and was reduced in Δ strain, while the hemicellulases and were increased in the presence of SCB. The mutation also affected the xyloglucan hydrolysis patterns. In addition, CEL74A activity was modulated in the presence of calcium, suggesting that this ion may be required for efficient degradation of xyloglucan.
Conclusions: CEL74A affects the regulation of holocellulolytic genes and the efficient degradation of SCB in . This data makes a significant contribution to our understanding of the carbon utilization of fungal strains as a whole.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123685 | PMC |
| http://dx.doi.org/10.3390/ijms22094545 | DOI Listing |
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Article Synopsis
- The filamentous fungus is important for producing enzymes used in biotechnology and has a complex system for breaking down biomass, but more research is needed on how its genes are regulated.
- A null mutant strain was created to study the effects of deleting the xyloglucanase gene, revealing that it impacts the expression of cellulases and hemicellulases during sugarcane bagasse cultivation.
- The findings suggest that the CEL74A enzyme plays a key role in regulating these genes and that calcium may enhance the fungus's ability to break down xyloglucan, improving our understanding of fungal carbon utilization.
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