Background: High-grade gliomas (HGGs) are highly malignant tumors with a poor survival rate. The inability of free drugs to cross the blood-brain barrier and their off-target accumulation result in dose-limiting side effects. This study aimed at enhancing the encapsulation efficiency (EE) of irinotecan hydrochloride trihydrate (IRH) within polycaprolactone (PCL) nanoparticles with optimized size and charge.
Materials And Methods: IRH-loaded PCL nanoparticles were formulated using either the single emulsion (O/W, W/O and O/O) or double emulsion (W/O/O and W/O/W) solvent evaporation techniques. The nanoparticles were characterized for their size, zeta potential and EE, with the optimized nanoparticles being characterized for their drug release and cytotoxicity.
Results: The amorphization of PCL and the addition of electrolytes to the aqueous phases of the W/O/W emulsion produced spherical nanoparticles with a mean diameter of 202.1 ± 2.0 nm and an EE of 65.0%. The IRH-loaded nanoparticles exhibited zero-order release and were cytotoxic against primary HGG cells.
Conclusion: The amorphization of PCL improves its EE of hydrophilic drugs, while the addition of electrolytes to the aqueous phases of the W/O/W emulsion enhances their EE further. IRH-loaded PCL nanoparticles have the potential to deliver cytotoxic levels of IRH over a sustained period of time, enhancing the cell death of HGGs.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068837 | PMC |
| http://dx.doi.org/10.3390/pharmaceutics13040541 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Label-free electrochemical biosensor based on AuNPs and PCL-b-PHEAA amphiphilic copolymer for highly sensitive detection of CEA.
Mikrochim Acta
March 2025
Pharmacy College, Henan University of Chinese Medicine, Zhengzhou, 450046, People's Republic of China.
Carcinoembryonic antigen (CEA) is a broad-spectrum tumor marker expressed in various human cancers. In this study, the electrode loaded with AuNPs was used to replace the traditional electrode, providing more active sites for biological probes and significantly enhancing the sensitivity of the sensor. By constructing an aptamer-antigen-aptamer "sandwich structure," highly selective detection was achieved, effectively avoiding cross-reactions.
View Article and Find Full Text PDFFabrication of a multilayer bioabsorbable composite vascular stent utilizing oxidized starch-FeO nanoparticles and polycaprolactone nanofibers.
Sci Rep
March 2025
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Currently, bioabsorbable polymer stents are utilized to mitigate the risk of late stent restenosis in cardiovascular applications. In this context, we propose a novel assembly that integrates polycaprolactone (PCL) nano-fibers and oxidized starch (OS) with iron oxide nanoparticles (IONPs) to create a rigid-swelling scaffold for cardiovascular stents. This study fabricates the nanocomposite (NC) using a combination of electrospinning and electrospray techniques.
View Article and Find Full Text PDFAdvanced amphotericin-B gel formulation: An efficient approach to combat cutaneous leishmaniasis.
Biomater Adv
February 2025
Graduate Program in Pharmaceutical Sciences, Department of Biochemistry and Pharmacology, Federal University of Piauí, Teresina, PI, Brazil.
Several drug release studies are continuously carried out to minimize pharmacological limitations, such as insolubility in water and gastrointestinal irritability. This study focused on the development of gels for the administration of amphotericin-B (AmB) for the treatment of cutaneous leishmaniasis. Two gels were prepared from copolymers (polyglycerol and ε-caprolactone) incorporated with AmB.
View Article and Find Full Text PDFElectrospun Collagen-Coated Nanofiber Membranes Functionalized with Silver Nanoparticles for Advanced Wound Healing Applications.
Membranes (Basel)
February 2025
Plastic and Reconstructive Surgery Unit, Cattinara Hospital, Strada di Fiume, 447, 34149 Trieste, Italy.
Complex wounds pose a significant healthcare challenge due to their susceptibility to infections and delayed healing. This study focuses on developing electrospun polycaprolactone (PCL) nanofiber membranes coated with Type I collagen derived from bovine skin and functionalized with silver nanoparticles (AgNPs) to address these issues. The collagen coating enhances biocompatibility, while AgNPs synthesized through chemical reduction with sodium citrate provide broad-spectrum antimicrobial properties.
View Article and Find Full Text PDFCorrigendum to "Polycaprolactone (PCL)-based films integrated with hairy cellulose nanocrystals and silver nanoparticles for active Tilapia packaging applications" [Food Chemistry X, 22 (2024), 101490].
Food Chem X
December 2024
Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
[This corrects the article DOI: 10.1016/j.fochx.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!