Cytotoxicity and apoptosis-inducing properties of compounds isolated from leaves were investigated. The hexane-soluble portion of MeOH extracts of leaves that showed cytotoxic activity was separated to yield seven compounds -. Chemical structure analysis using NMR spectroscopy and mass spectrometry confirmed that compound was canophyllol, and compounds - were garcinielliptones N, O, J, G, F, and garcinielliptin oxide, respectively. Among them, garcinielliptone G () showed growth inhibition by causing apoptosis in THP-1 and Jurkat cells derived from human acute monocytic leukemia and T lymphocyte cells, respectively. Apoptosis induced by garcinielliptone G () was demonstrated by the detection of early apoptotic cells with fluorescein-labeled Annexin V and increases in cleaved caspase-3 and cleaved PARP protein levels. However, the addition of caspase inhibitor Z-VAD-FMK did not affect growth arrest or apoptosis induction. These results suggest that garcinielliptone G () can induce both caspase-3 activation and caspase-independent apoptosis. Therefore, garcinielliptone G () may be a potential candidate for acute leukemia treatment.
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