has caused high rates of mortality due to the appearance of strains with multidrug resistance (MDR) profiles. This study aimed to characterize the molecular profile of virulence and resistance genes in 99 isolates of recovered from different clinical specimens. The isolates were identified by the automated method Vitek2, and the antibiotic susceptibility profile was determined using different classes of antimicrobials. The genomic DNA was extracted and amplified by multiplex polymerase chain reaction (mPCR) to detect different virulence and antimicrobial resistance genes. Molecular typing was performed using the enterobacterial repetitive intergenic consensus (ERIC-PCR) technique to determine the clonal relationship among isolates. The drug susceptibility profiles of for all strains showed high levels of drug resistance, particularly, 27 (27.3%) isolates that exhibited extensively drug-resistant (XDR) profiles, and the other isolates showed MDR profiles. We detected the polymyxin E () gene in one strain that showed resistance against colistin. The genes that confer resistance to oxacillin ( and ) were present in three isolates. One of these isolates carried both genes. As far as we know from the literature, this is the first report of the presence of and genes in .
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069495 | PMC |
http://dx.doi.org/10.3390/microorganisms9040786 | DOI Listing |
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