AI Article Synopsis
- Bone marrow mesenchymal stem cells (MSCs) are valuable in clinical settings but face challenges due to their diverse functions and cellular variability.
- Research using single-cell RNA sequencing revealed that fatty acid pathways, particularly butyrate, play a key role in influencing MSC behavior and differentiation.
- While butyrate reduced MSC self-renewal and differentiation, it also enhanced the expression of factors that support hematopoietic stem cell (HSC) niche function, suggesting its potential as a therapeutic tool.
Article Abstract
Bone marrow mesenchymal stem cells (MSCs) are widely used clinically due to their versatile roles in multipotency, immunomodulation, and hematopoietic stem cell (HSC) niche function. However, cellular heterogeneity limits MSCs in the consistency and efficacy of their clinical applications. Metabolism regulates stem cell function and fate decision; however, how metabolites regulate the functional heterogeneity of MSCs remains elusive. Here, using single-cell RNA sequencing, we discovered that fatty acid pathways are involved in the regulation of lineage commitment and functional heterogeneity of MSCs. Functional assays showed that a fatty acid metabolite, butyrate, suppressed the self-renewal, adipogenesis, and osteogenesis differentiation potential of MSCs with increased apoptosis. Conversely, butyrate supplement significantly promoted HSC niche factor expression in MSCs, which suggests that butyrate supplement may provide a therapeutic approach to enhance their HSC niche function. Overall, our work demonstrates that metabolites are essential to regulate the functional heterogeneity of MSCs.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075002 | PMC |
| http://dx.doi.org/10.3389/fcell.2021.653308 | DOI Listing |
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