AI Article Synopsis
- West Nile virus (WNV) is deadly to humans and horses and requires a precise diagnostic system due to cross-reactivity with the Japanese encephalitis virus (JEV).
- Research focused on how deleting the C-terminal region of the WNV E protein affects the production of subviral particles (SPs) and the antigenicity of the E protein.
- The study found that while deletion suppresses SP formation, full-length E protein is more effective in ELISA tests, showing less cross-reactivity with JEV antibodies and similar sensitivity to neutralization tests.
Article Abstract
West Nile virus (WNV), a member of the Japanese encephalitis virus (JEV) serocomplex group, causes lethal encephalitis in humans and horses. Because serodiagnosis of WNV and JEV is hampered by cross-reactivity, the development of a simple, secure, and WNV-specific serodiagnostic system is required. The coexpression of prM protein and E protein leads to the secretion of subviral particles (SPs). Deletion of the C-terminal region of E protein is reported to affect the production of SPs by some flaviviruses. However, the influence of such a deletion on the properties and antigenicity of WNV E protein is unclear. We analyzed the properties of full-length E protein and E proteins lacking the C-terminal region as novel serodiagnostics for WNV infection. Deletion of the C-terminal region of E protein suppressed the formation of SPs but did not affect the production of E protein. The sensitivity of an enzyme-linked immunosorbent assay (ELISA) using the full-length E protein was higher than that using the truncated E proteins. Furthermore, in the ELISA using full-length E protein, there was little cross-reactivity with anti-JEV antibodies, and the sensitivity was similar to that of the neutralization test.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080695 | PMC |
| http://dx.doi.org/10.1038/s41598-021-88777-5 | DOI Listing |
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