New oxindole alkaloids with selective osteoclast inhibitory activity from .

A new alkaloid 14-hydroxygelseziridine (), along with four known oxindoles (), was isolated and characterized from the well-known toxic medicine . Their structures were elucidated by means of spectroscopic techniques and quantum chemistry calculations. Structurally, new compound has a three membered oxygen ring at -4/C-20. All compounds were tested for osteoclast (MOC-1) inhibitory activity . Compound exhibited the selective osteoclast inhibitory activity. Flow cytometry revealed that the apoptosis of osteoclasts induced by . Furthermore, the PCR bioassay suggested that compound may activate the apoptotic pathway of osteoclasts by reducing the expression of IL-6 and c-Jun, and increasing caspase 9. This work provided the evidence for the rationality as the traditional treatment for bone related diseases of , and shed a new light on its further research.