AI Article Synopsis
- Changes in gut microbiota are linked to the growth of brain tumors and the effects of temozolomide (TMZ) therapy, but the details of these changes are not fully understood.
- An experiment with mice showed distinct alterations in gut microbiota during glioma progression, particularly affecting the Firmicutes and Bacteroidetes phyla.
- TMZ treatment resulted in specific increases in beneficial gut microbes, which may enhance its anti-tumor effects, suggesting a connection between gut health and cancer treatment outcomes.
Article Abstract
Background: Gut microbiota is associated with the progression of brain tumors. However, the alterations in gut microbiota observed during glioma growth and temozolomide (TMZ) therapy remain poorly understood.
Methods: C57BL/6 male mice were implanted with GL261 glioma cells. TMZ/sodium carboxymethyl cellulose (SCC) was administered through gavage for five consecutive days (from 8 to 12 days after implantation). Fecal samples were collected before (T0) and on days 7 (T1), 14 (T2), and 28 (T3) after implantation. The gut microbiota was analyzed using 16S ribosomal DNA sequencing followed by absolute and relative quantitation analyses.
Results: Nineteen genera were altered during glioma progression with the most dramatic changes in Firmicutes and Bacteroidetes phyla. During glioma growth, abundance decreased in the early stage (T1) and then gradually increased (T2, T3); abundance exhibited a persistent increase; showed a transient increase (T2) and then a subsequent decrease (T3). Similar longitudinal changes in and abundance were observed in TMZ-treated mice, but the decrease of at T3 in the TMZ-treated group was less than that in the vehicle-treated group. No significant change in was observed after TMZ treatment. Additionally, compared to vehicle control, TMZ treatment led to an enrichment in and .
Conclusion: Glioma development and progression altered the composition of gut microbiota. Induction of and as well as the prevention of the reduction in may contribute to the anti-tumor effect of TMZ. This study helps to reveal the association between levels of specific microbial species in the gut and the anti-tumor effect of TMZ.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071088 | PMC |
| http://dx.doi.org/10.2147/DDDT.S298261 | DOI Listing |
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