AI Article Synopsis

  • The primary diagnosis of SARS-CoV-2 relies on detecting viral RNA in nasal swabs, while analyzing antibodies plays a crucial role in diagnostics and estimating prevalence.
  • Researchers developed enzyme immunoassays (EIA) to detect antibodies against different SARS-CoV-2 proteins and compared neutralizing activity with traditional IgE test results.
  • The study found that using N and RBD proteins improved sensitivity for detecting immune responses in COVID-19 patients, indicating that combined analysis of specific antibodies can enhance serological diagnostics for COVID-19.

Article Abstract

Background: Primary diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is based on detection of virus RNA in nasopharyngeal swab samples. In addition, analysis of humoral immunity against SARS-CoV-2 has an important role in viral diagnostics and seroprevalence estimates.

Methods: We developed and optimized an enzyme immunoassays (EIA) using SARS-CoV-2 nucleoprotein (N), S1 and receptor binding domain (RBD) of the viral spike protein, and N proteins from SARS, Middle East respiratory syndrome (MERS), and 4 low-pathogenic human CoVs. Neutralizing antibody activity was compared with SARS-CoV-2 IgG, IgA, and IgM EIA results.

Results: The sensitivity of EIA for detecting immune response in COVID-19 patients (n = 101) was 77% in the acute phase and 100% in the convalescent phase of SARS-CoV-2 infection when N and RBD were used as antigens in IgG and IgA specific EIAs. SARS-CoV-2 infection significantly increased humoral immune responses against the 229E and NL63 N proteins. S1 and RBD-based EIA results had a strong correlation with microneutralization test results.

Conclusions: The data indicate a combination of SARS-CoV-2 S1 or RBD and N proteins and analysis of IgG and IgA immunoglobulin classes in sera provide an excellent basis for specific and sensitive serological diagnostics of COVID-19.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8135300PMC
http://dx.doi.org/10.1093/infdis/jiab222DOI Listing

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