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Filename: Session/Session.php
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Filename: controllers/Detail.php
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Function: _error_handler
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Function: insertAPISummary
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Phosphorylation of the histone protein H2AX to form γ-H2AX foci directly represents DNA double-strand break formation. Traditional γ-H2AX detection involves counting individual foci within individual nuclei. The novelty of this work is the application of a time-resolved fluorescence assay using dissociation-enhanced lanthanide fluorescence immunoassay for quantitative measurements of γ-H2AX. For comparison, standard fluorescence detection was employed and analyzed either by bulk fluorescent measurements or by direct foci counting using BioTek Spot Count algorithm and Gen 5 software. Etoposide induced DNA damage in A549 carcinoma cells was compared across all test platforms. Time resolved fluorescence detection of europium as a chelated complex enabled quantitative measurement of γ-H2AX foci with nanomolar resolution. Comparative bulk fluorescent signals achieved only micromolar sensitivity. Lanthanide based immunodetection of γ-H2AX offers superior detection and a user-friendly workflow. These approaches have the potential to improve screening of compounds that either enhance DNA damage or protect against its deleterious effects.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076281 | PMC |
http://dx.doi.org/10.1038/s41598-021-88296-3 | DOI Listing |
Int J Biol Macromol
December 2024
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address:
Bovine serum albumin (BSA) is one of the most abundant proteins in serum, and its high-throughput detection is still one of the current challenges. Nitrogen‑phosphorus co-doped carbon dots (CDs) were synthesized by a hydrothermal method. Adenosine monophosphate (AMP) was used as a precursor for the synthesis of CDs, providing the required carbon, nitrogen and phosphorus sources for the CDs.
View Article and Find Full Text PDFTalanta
December 2024
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science & Engineering, Shanghai Institute of Pollution Control and Ecological Security, Tongji University, 1239 Siping Road, Shanghai, 200092, China. Electronic address:
The excessive presence of the metal ions Cu and Fe in the environment poses a serious threat to ecosystems and human health, so timely and accurate detection of them has become essential and urgent. In this paper, a novel hydrogel-based fluorescent sensor, named ME-IPA@SA-TbZn, was fabricated facilely through an in-situ cross-linking modification method and was used for the detection of Cu and Fe in water bodies. The ME-IPA@SA-TbZn is essentially a hybrid hydrogel bead that exhibits vibrant fluorescence, employing Tb and Zn functionalized hydrogen-bonded organic frameworks (HOFs) as the fluorescence functional core and sodium alginate (SA) as the hydrogel matrix.
View Article and Find Full Text PDFMikrochim Acta
December 2024
Department of Chemistry, College of Science, University of Sulaimani, Qliasan St. 46002, Sulaymaniyah, Kurdistan Region, Iraq.
An efficient dual-state blue-emitting zinc-based metal-organic frameworks (MOFs), designated as UoZ-8 has been developed. Coordination-induced emission causes the UoZ-8 to give the blue emission in both solid and dispersed form in liquid. Upon the addition of tetracycline (TC), a noticeable shift from blue emission to greenish-yellow emission occurred, with a marked increase in intensity, which was attributed to the inner filter effect accompanied by aggregation-induced emission (IFE-AIE).
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
December 2024
Department of Chemistry, School of Physical and Mathematical Sciences, University of Kerala, Kariavattom Campus, Thiruvananthapuram 695581, Kerala, India; International Inter-University Centre for Sensing and Imaging (IIUCSI), Department of Chemistry, University of Kerala, Kariavattom Campus, Thiruvananthapuram 695581, Kerala, India. Electronic address:
Bilirubin is an important biomarker indicative of human health metabolism, especially in jaundice disease. In this work, we develop an Eu (Europium)-tungstate complex-based turn-off luminescence detection method for bilirubin in human (urineand serum)samples. The Eu-tungstate complex was synthesized by a straightforward one-pot procedure at ambient temperature, utilizing Europium chloride and Sodium tungstate as the precursors.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
December 2024
Department of Chemical Engineering, The City College of New York, New York, NY 10031.
Rare earth elements (REEs) are critical materials to modern technologies. They are obtained by selective separation from mining feedstocks consisting of mixtures of their trivalent cation. We are developing an all-aqueous, bioinspired, interfacial separation using peptides as amphiphilic molecular extractants.
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