[Figure: see text].
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1161/CIRCRESAHA.120.318234 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Chronic GIPR agonism results in pancreatic islet GIPR functional desensitisation.
Mol Metab
January 2025
Section of Endocrinology and Investigative Medicine, Imperial College London, United Kingdom. Electronic address:
Objective: There is renewed interest in targeting the glucose-dependent insulinotropic polypeptide receptor (GIPR) for treatment of obesity and type 2 diabetes. G-protein coupled receptor desensitisation is suggested to reduce the long-term efficacy of glucagon-like-peptide 1 receptor (GLP-1R) agonists and may similarly affect the efficacy of GIPR agonists. We explored the extent of pancreatic GIPR functional desensitisation with sustained agonist exposure.
View Article and Find Full Text PDFStackable Guides: Digital Workflows for Full Arch Dental Implant Rehabilitation.
Oral Maxillofac Surg Clin North Am
January 2025
Department of Maxillofacial Prosthetics and Prosthodontics, Naval Medical Center San Diego California, USA.
Computer-guided full-arch dental rehabilitation, which is highly technique-sensitive, benefits from advancements in 3-dimensional-printed guides and open-source software. Critical pre-surgical planning includes patient selection, health history evaluations, and detailed imaging like cone beam computed tomography scans. Various surgical guides-freehand, static, dynamic, stackable, and interchangeable-are used based on case complexity, with stackable guides improving workflow efficiency.
View Article and Find Full Text PDFNeurochemical signals like dopamine (DA) play a crucial role in a variety of brain functions through intricate interactions with other neuromodulators and intracellular signaling pathways. However, studying these complex networks has been hindered by the challenge of detecting multiple neurochemicals simultaneously. To overcome this limitation, we developed a single-protein chemigenetic DA sensor, HaloDA1.
View Article and Find Full Text PDFExcitable Ras dynamics-based screens reveal RasGEFX is required for macropinocytosis and random cell migration.
Nat Commun
January 2025
Laboratory of Single Molecule Biology, Graduate School of Science and Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Excitable systems of eukaryotic chemotaxis can generate asymmetric signals of Ras-GTP-enriched domains spontaneously to drive random cell migration without guidance cues. However, the molecules responsible for the spontaneous signal generation remain elusive. Here, we characterized RasGEFs encoded in Dictyostelium discoideum by live-cell imaging of the spatiotemporal dynamics of Ras-GTP and hierarchical clustering, finding that RasGEFX is primarily required for the spontaneous generation of Ras-GTP-enriched domains and is essential for random migration in combination with RasGEFB/M/U in starved cells, and they are dispensable for chemotaxis to chemoattractant cAMP.
View Article and Find Full Text PDFCytosolic-enhanced dark Epac-based FRET sensors allow for intracellular cAMP detection in live cells via FLIM.
FEBS Lett
December 2024
Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
Fluorescence resonance energy transfer (FRET)-based biosensors are powerful tools for studying second messengers with high temporal and spatial resolution. FRET is commonly detected by ratio imaging, but fluorescence lifetime imaging microscopy (FLIM), which measures the donor fluorophore's lifetime, offers a robust and more quantitative alternative. We have introduced and optimized four generations of FRET sensors for cAMP, based on the effector molecule Epac1, including variants for either ratio imaging or FLIM detection.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!