Super-resolution microscopy (SRM) imaging of the finite subcellular structures and subtle bioactivities inside organelles delivers abundant cellular information with high fidelity to unravel the intricate biological processes. An ideal fluorescent probe with precise control of fluorescence is critical in SRM technique like stimulated emission depletion (STED). Si-rhodamine was decorated with both targeting group and H -receptor, affording the dually fluorogenic Si-rhodamine in which the NIR fluorescence was efficiently controlled by the coalescent of spirolactone-zwitterion equilibrium and PeT mechanism. The dually fluorogenic characters of the probe offer a perfect mutual enhancement in sensitivity, specificity and spatial resolution. Strong fluorescence only released in the existence of targeting protein at acidic lysosomal pH, ensured precisely tracking the dynamic of lysosomal structure and pH in living cells by STED.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/chem.202100623 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Nitrile-aminothiol bioorthogonal near-infrared fluorogenic probes for ultrasensitive in vivo imaging.
Nat Commun
January 2025
State Key Laboratory of Anti-Infective Drug Discovery and Development, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China.
Bioorthogonal chemistry-mediated self-assembly holds great promise for dynamic molecular imaging in living organisms. However, existing approaches are limited to nanoaggregates with 'always-on' signals, suffering from high signal-to-background ratio (SBR) and compromised detection sensitivity. Herein we report a nitrile-aminothiol (NAT) bioorthogonal fluorogenic probe (CyNA-SS-FK) for ultrasensitive diagnosis of orthotopic hepatocellular carcinoma.
View Article and Find Full Text PDFMechanical tough and multicolor aggregation-induced emissive polymeric hydrogels for fluorescent patterning.
Nanoscale Adv
January 2023
Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences Ningbo 315201 China
Aggregation-induced emissive fluorogens (AIEgens) are promising building blocks for fluorescent polymeric hydrogels (FPHs) because intense fluorescence intensities are usually guaranteed by spontaneous aggregates of hydrophobic AIEgens in a hydrophilic polymer network. However, most AIE-active FPHs are single-color fluorescent and cannot display tunable emission colors. Additionally, efforts to produce mechanically strong AIE-active hydrogels have been largely ignored, restricting their potential uses.
View Article and Find Full Text PDFSTED Imaging the Dynamics of Lysosomes by Dually Fluorogenic Si-Rhodamine.
Chemistry
July 2021
Department of Organic Chemistry, College of Pharmacy, Second Military Medical University, Shanghai, 200433, P. R. China.
Super-resolution microscopy (SRM) imaging of the finite subcellular structures and subtle bioactivities inside organelles delivers abundant cellular information with high fidelity to unravel the intricate biological processes. An ideal fluorescent probe with precise control of fluorescence is critical in SRM technique like stimulated emission depletion (STED). Si-rhodamine was decorated with both targeting group and H -receptor, affording the dually fluorogenic Si-rhodamine in which the NIR fluorescence was efficiently controlled by the coalescent of spirolactone-zwitterion equilibrium and PeT mechanism.
View Article and Find Full Text PDFSalmon-derived thrombin inhibits development of chronic pain through an endothelial barrier protective mechanism dependent on APC.
Biomaterials
February 2016
Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Neurosurgery, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address:
Many neurological disorders are initiated by blood-brain barrier breakdown, which potentiates spinal neuroinflammation and neurodegeneration. Peripheral neuropathic injuries are known to disrupt the blood-spinal cord barrier (BSCB) and to potentiate inflammation. But, it is not known whether BSCB breakdown facilitates pain development.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!