Evaluating DNA damage response through immunofluorescence staining of primordial germ cells in L1 larva.

L1 larvae have two well-defined primordial germ cells embedded in a niche comprising two somatic gonad precursor cells. Thus, provides an ideal model for studying intercellular signaling in response to DNA damage. However, existing staining protocols are focused on worms in later developmental stages and are not optimized for the L1 larvae. Here, we present a revised protocol for assessing the DNA damage response utilizing immunofluorescence staining specifically in L1 larva. For complete details on the use and execution of this protocol, please refer to Ou et al. (2019).