is a diplomonad genus known to inhabit the intestinal tracts of rodents. Ultrastructural morphology and 18S rDNA gene sequence analysis support the placement of as the closest sister lineage to , a parasite which causes diarrheal disease in humans and animals worldwide. However, further information on the ecology and diversity of is currently scarce. Expanding the available database of characterized sequences for this organism would therefore be helpful to studies of Diplomonad ecology, evolution, and epidemiology, particularly related to the evolution of parasitism in and another related Diplomonad common in commercial fish farming. In order to study the prevalence and genotypic diversity of , we developed a nested PCR assay specific to and optimized to detect genotypes in fecal samples collected from wildlife in a New York watershed, and sequenced a portion of the small subunit ribosomal DNA (18S rDNA) gene to identify samples to species level. Molecular evidence suggested that genotypes display similar prevalence to and microsporidian pathogens in wildlife as well as strong host preference for rodent and opossum hosts. Phylogenetic analysis showed strong support for 14 genotypes, 13 of these novel, and patterns of host-parasite co-evolution.
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2024 taxonomic update for the families Naryaviridae, Nenyaviridae, and Vilyaviridae.
Arch Virol
December 2024
Institut Pasteur, Université Paris Cité, CNRS UMR6047, Archaeal Virology Unit, Paris, France.
The families Naryaviridae (order Rivendellvirales), Nenyaviridae (order Rohanvirales), and Vilyaviridae (order Cirlivirales), all within the class Arfiviricetes of the phylum Cressdnaviricota, include single-stranded DNA viruses associated with protozoan parasites of the genera Entamoeba and Giardia as well as viruses found in various environmental samples, also likely infecting protozoans. Here, we provide a taxonomic update for these three families, which were recently expanded with multiple new members. In particular, we established seven new genera and nine new species in the family Naryaviridae, one new genus with one new species in the family Nenyaviridae, and three new genera and nine new species in the family Vilyaviridae.
View Article and Find Full Text PDFHost-specific , and in shelter dogs from central Europe.
Parasitology
April 2024
Department of Biology and Medical Parasitology, Wroclaw Medical University, Wroclaw, Poland.
spp., and microsporidia are unicellular opportunistic pathogens that can cause gastrointestinal infections in both animals and humans. Since companion animals may serve as a source of infection, the aim of the present screening study was to analyse the prevalence of these intestinal protists in fecal samples collected from dogs living in 10 animal shelters in central Europe (101 dogs from Poland and 86 from the Czech Republic), combined with molecular subtyping of the detected organisms in order to assess their genetic diversity.
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Eur J Protistol
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Department of Zoology, Faculty of Science, Charles University, Viničná 7, Prague 128 00, Czech Republic. Electronic address:
Diplomonadida is a lineage of anaerobic protists belonging to Fornicata, Metamonada. Most diplomonads are endobiotic or parasitic, such as Giardia intestinalis, which is a famous human pathogen, but several free-living species exist as well. Although it has been proposed that the free-living diplomonads are descendants of endobiotic organisms and thus interesting from the evolutionary point of view, they have been largely neglected.
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Infect Genet Evol
September 2023
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450046, China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou, Henan 450046, China; Key Laboratory of Quality and Safety Control of Poultry Products (Zhengzhou), Ministry of Agriculture and Rural Affairs, Zhengzhou, Henan 450046, China. Electronic address:
Article Synopsis
- Giardia is a type of parasite that lives in the small intestine of humans and animals, leading to a condition called giardiasis, which is characterized by diarrhea.
- The genetic diversity among different Giardia isolates affects how the disease spreads and how it manifests in individuals, influenced by the relationship with their hosts.
- Modern molecular diagnostic techniques allow scientists to study this genetic diversity, improving knowledge about Giardia's population structure and transmission routes, as well as the clinical aspects of giardiasis.
Combined nanometric and phylogenetic analysis of unique endocytic compartments in Giardia lamblia sheds light on the evolution of endocytosis in Metamonada.
BMC Biol
September 2022
Institute of Cell Biology, University of Bern, Bern, Switzerland.
Background: Giardia lamblia, a parasitic protist of the Metamonada supergroup, has evolved one of the most diverged endocytic compartment systems investigated so far. Peripheral endocytic compartments, currently known as peripheral vesicles or vacuoles (PVs), perform bulk uptake of fluid phase material which is then digested and sorted either to the cell cytosol or back to the extracellular space.
Results: Here, we present a quantitative morphological characterization of these organelles using volumetric electron microscopy and super-resolution microscopy (SRM).
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