Polymorphisms in xenobiotic metabolism-related genes in patients with hepatocellular carcinoma: a case-control study.

This study was performed to investigate the relationship between polymorphisms in microsomal epoxide hydrolase (; Tyr113His and His139Arg substitution) and glutathione S-transferase (; deletion, deletion, and .Ala114Val substitution) and their correlation with clinico-histopathological features in hepatocellular carcinoma (HCC).We evaluated environmental risk factors and genetic alterations in 556 individuals (86 cases and 470 controls). PCR multiplex for and , polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) for , and real-time PCR for were performed. Statistical analyses were performed using multiple logistic regression tests.Age over 48 years ( < 0.001) and alcohol consumption ( = 0.021) were the predictors of increased risk of developing HCC. .Ala114Val for all regression models ( < 0.05), except the recessive model, and the null genotype (odds ratio [OR] = 0.43, 95% confidence interval [CI] = 0.21-0.87,  = 0.019) were predictors of an increased risk of developing HCC. Polymorphic , Ala114Val, and .His139Arg and wild-type mEH.Tyr113His (OR = 5.04; 95% CI = 1.59-16.04;  = 0.006) were associated with HCC.Age over 48 years, alcohol consumption, and the presence of polymorphic variants of and were associated with the risk of developing HCC.