AI Article Synopsis
- Phase-contrast synchrotron-based X-ray imaging, using an X-ray interferometer, offers high sensitivity and resolution to visualize the detailed structures of biological soft tissues, including tumors.
- This study compared images from this advanced X-ray technique with traditional histopathological methods in rat testicular tumors, confirming that X-ray imaging can reliably identify cancer cell characteristics based on nuclear chromatin density.
- The technology allows for 3D imaging of tumors, revealing different tissue types and structures with a spatial resolution as fine as 26 μm, highlighting its potential for preclinical cancer research.
Article Abstract
Phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer provides high sensitivity and high spatial resolution, and it has the ability to depict the fine morphological structures of biological soft tissues, including tumors. In this study, we quantitatively compared phase-contrast synchrotron-based X-ray computed tomography images and images of histopathological hematoxylin-eosin-stained sections of spontaneously occurring rat testicular tumors that contained different types of cells. The absolute densities measured on the phase-contrast synchrotron-based X-ray computed tomography images correlated well with the densities of the nuclear chromatin in the histological images, thereby demonstrating the ability of phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer to reliably identify the characteristics of cancer cells within solid soft tissue tumors. In addition, 3-dimensional synchrotron-based phase-contrast X-ray computed tomography enables screening for different structures within tumors, such as solid, cystic, and fibrous tissues, and blood clots, from any direction and with a spatial resolution down to 26 μm. Thus, phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer shows potential for being useful in preclinical cancer research by providing the ability to depict the characteristics of tumor cells and by offering 3-dimensional information capabilities.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085371 | PMC |
| http://dx.doi.org/10.1177/15330338211010121 | DOI Listing |
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