AI Article Synopsis
- - The study aimed to evaluate the effects of benznidazole (BZ) treatment on mice infected with different forms of Trypanosoma cruzi, a parasite causing Chagas disease.
- - Swiss mice were divided into two groups and inoculated with either blood or insect-derived forms of the parasite, with half of each group receiving BZ treatment and the other half serving as untreated controls.
- - Results showed that while the blood-inoculated mice had a quicker and more intense infection, neither group responded to BZ treatment, indicating the infective form impacts infection outcome, but not treatment effectiveness.
Article Abstract
Purpose: Our goal was to analyze the outcome of infection and response to benznidazole (BZ) treatment in mice intragastrically inoculated with trypomastigotes forms of Trypanosoma cruzi from different origins.
Methods: Twenty-four Swiss mice were divided in two groups and inoculated, by gavage, with 1 × 10 blood trypomastigotes (BT) or insect-derived metacyclic trypomastigotes (IT) of AM14 strain (T. cruzi IV). Half of the animals of each group were treated with BZ (TBZ), from 10 to 30th days after the inoculation, and the other constituted the untreated control groups (NT). After the etiological treatment, all mice were immunosuppressed with cyclophosphamide for three weeks. Parasitological and molecular parameters, infectivity, cumulative mortality, and reactivation post-immunosuppression rates were obtained.
Results: Animals inoculated with BT showed lower pre-patent period and early day of the maximum parasitemia, as well as a higher maximum peak of parasitemia than the IT animals. However, both, BT and IT animals, did not respond to BZ treatment (0.0% of cure).
Conclusion: We conclude that the infective form influences in the outcome of infection, but not the response to the etiological treatment in mice intragastrically infected with the T. cruzi IV strain studied.
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| http://dx.doi.org/10.1007/s11686-021-00393-5 | DOI Listing |
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