A bifunctional fluorescent probe for simultaneous detection of GSH and HS (n > 1) from different channels with long-wavelength emission.

In this work, we presented a long-wavelength emission fluorescent probe DCM-Cou-SePh that can discriminatively detect glutathione (GSH) and hydrogen polysulfides (HS, n > 1) from green and red emission channels, respectively. With the addition of GSH, probe DCM-Cou-SePh displayed green fluorescence emission (λ = 430/530 nm). In the presence of HS, the probe exhibited a significant fluorescence enhancement in red channel (λ = 560/680 nm). We also demonstrated that this probe was suitable to quantitatively detect GSH and HS with low detection limits (0.12 μM for GSH, 0.19 μM for HS). Furthermore, DCM-Cou-SePh can be used for sensing endogenous GSH and HS in living cells by dual-color fluorescence imaging.