Four groups of cows, each group comprising from 5,000 to 5,500 animals, were inseminated with 7.5, 10, 15 and 20 millions of living and dead spermatozoa respectively. This was deep frozen semen in 0.25 ml straws. The fertilisation rates of the groups did not differ significantly, even when the fertility levels of the twenty-eight bulls involved were taken into account.
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