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Contribution of the classical polymerase chain reaction in the diagnosis of a HIV-1 infected patient in Benin: a case report. | LitMetric

Contribution of the classical polymerase chain reaction in the diagnosis of a HIV-1 infected patient in Benin: a case report.

AIDS Res Ther

Laboratory of Cell Biology and Physiology, Department of Biochemistry and Cellular Biology, Faculty of Sciences and Technology (FAST), Institute of Applied Biomedical Sciences (ISBA), University of Abomey-Calavi, Calavi, 01 BP 918, Cotonou, Benin.

Published: April 2021

Background: First ambitious target by 2020 of UNAIDS is that 90% of people living with HIV know their HIV status. In people older than 18 months of age, serological confirmation test is recommended to confirm HIV infection.

Case Presentation: Here we report the case of a patient tested positive with HIV-1, ELISA, Murex Ag⁄Ab Combination assay (OD450 = 0.802 and cutoff-OD = 0.279) and negative by using FIRST RESPONSE HIV1-2.O CARD TEST (version 2.0) RAPID HIV CARD TEST. Viral load performed with Cobas TaqMan 96/Cobas Ampliprep was 6.49log The virus could be sequenced in partial gag and pol genes and belonged to CRF02_AG clade.

Conclusion: Conventional PCR is a complementary method for the diagnosis of inconclusive HIV-1 serologies by antibodies.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059016PMC
http://dx.doi.org/10.1186/s12981-021-00342-2DOI Listing

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