Pulse dipolar spectroscopy (PDS) in Electron Paramagnetic Resonance (EPR) is the method of choice for determining the distance distribution function for mono-, bi- or multi- spin-labeled macromolecules and nanostructures. PDS acquisition schemes conventionally use uniform sampling of the dipolar trace, but non-uniform sampling (NUS) schemes can decrease the total measurement time or increase the accuracy of the resulting distance distributions. NUS requires optimization of the data acquisition scheme, as well as changes in data processing algorithms to accommodate the non-uniformly sampled data. We investigate in silico the applicability of the NUS approach in PDS, considering its effect on random, truncation and sampling noise in the experimental data. Each type of noise in the time-domain data propagates differently and non-uniformly into the distance spectrum as errors in the distance distribution. NUS schemes seem to be a valid approach for increasing sensitivity and/or throughput in PDS by decreasing and redistributing noise in the distance spectrum so that it has less impact on the distance spectrum.
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PLoS One
January 2025
Sensory Circuits and Neurotechnology Laboratory, The Francis Crick Institute, London, United Kingdom.
Odours released by objects in natural environments can contain information about their spatial locations. In particular, the correlation of odour concentration timeseries produced by two spatially separated sources contains information about the distance between the sources. For example, mice are able to distinguish correlated and anti-correlated odour fluctuations at frequencies up to 40 Hz, while insect olfactory receptor neurons can resolve fluctuations exceeding 100 Hz.
View Article and Find Full Text PDFJ Ophthalmic Inflamm Infect
January 2025
School of medicine, Shahid Sadoughi University of Medical sciences, Yazd, Iran.
Introduction: Infectious keratitis is a rare but devastating complication following photorefractive keratectomy (PRK) that may lead to visual impairment. This study assessed the clinical features, treatment strategies, and outcomes of post-PRK infectious keratitis.
Methods: This retrospective study was conducted on patients with post-PRK infectious keratitis presenting to Khalili Hospital, Shiraz, Iran, from June 2011 to March 2024.
Surgery
January 2025
Experimental Surgery and Simulation Center, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile; Department of Digestive Surgery, Pontificia Universidad Católica de Chile, Santiago, Chile. Electronic address:
Distance and remote simulation have emerged as vital tools in modern surgical education, offering solutions to challenges such as limited operating hours, growing clinical demands, and the need for consistent, high-quality training. This review examines the benefits, limitations, and strategies for implementing sustainable distance simulation, structured around 3 foundational pillars: (1) effective hardware and infrastructure, including simulators and realistic scenarios that enable trainees to develop essential skills; (2) validated training programs grounded in educational theory with a clear focus on skill transfer and predictive validity; and (3) timely access to effective feedback. Distance simulation permits adaptable, scalable training environments, but the addition of remote and deferred feedback has further broadened its impact, helping to overcome the challenges posed by faculty availability and clinician time constraints.
View Article and Find Full Text PDFAdv Sci (Weinh)
January 2025
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea.
Non-close-packed crystalline arrays of colloidal particles in an elastic matrix exhibit mechanochromism. However, small interparticle distances often limit the range of reversible color shifts and reduce reflectivity during a blueshift. A straightforward, reproducible strategy using matrix swelling to increase interparticle distance and improve mechanochromic performance is presented.
View Article and Find Full Text PDFJ Appl Clin Med Phys
January 2025
University Clinic for Medical Radiation Physics, Medical Campus Pius Hospital, Carl von Ossietzky University, Oldenburg, Germany.
Purpose: The self-shielding radiosurgery system ZAP-X consists of a 3 MV linear accelerator and eight round collimators. For this system, it is a common practice to perform the reference dosimetry using the largest 25 mm diameter collimator at a source-to-axis distance (SAD) of 45 cm with the PTW Semiflex3D chamber placed at a measurement depth of 7 mm in water. Existing dosimetry protocols do not provide correction for these measurement conditions.
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