Change of transcription level of photoreceptor-specific CRX gene in the peripheral blood of the participants of an arctic world oceanic international flight.

Unlabelled: The gene encoding the cone-rod homeobox protein is a specific photoreceptor transcription factor crucial for retinal function. Persons temporarily residing in the Arctic zone during the polar summer may suffer from disturbances associated with extremely high ambient illumination. These environmental changes are mediated by retinal photoreceptors; therefore, it is important to study the expression of retinal genes in order to assess individual capacities of sensory adaptation to polar day conditions.

Purpose: To reveal the dynamics of expression level in humans after a prolonged temporary exposure to polar day conditions.

Material And Methods: The study included 6 pilots (males from 39 to 69 y.o.) who participated in the Arctic World Oceanic International Flight (West to East, from 62°N 74°E to 72°N 114°E). Samples of peripheral blood for RNA isolation were collected at the start and at the end of the flight. The level of mRNA in the samples was evaluated based on the data of quantitative real-time PCR of the gene, as well as the and housekeeping genes (reference).

Results: Expression of the gene in the studied group (<0.01) was revealed; the total average level of mRNA was about 3 times higher prior to, and approximately 7 times higher after normalization to the gene. Five pilots had increased expression of the gene within the range of -1.53 to -3.07 (Z-score of <0 before the flight and >0 after the flight). In one pilot, the level of expression was higher at the start, but it reduced by the end of the circumnavigation flight.

Conclusions: The results confirm the hypothesis that the gene is expressed after a prolonged temporary exposure to polar day conditions. It was also revealed that during rapid adaptation, equal changes in the illumination of retinal photoreceptors lead to different individual dynamics of expression.