AI Article Synopsis
- The study investigates how glycine and TNF-α receptors affect the release of pro-inflammatory proteins in 3T3-L1 fat cells.
- Glycine receptors were identified in these cells, and experiments showed that glycine can reduce levels of TNF-α and IL-6, but this effect depends on the presence of the TNF-α receptor.
- The results suggest that glycine might reduce inflammation by interfering with TNF-α signaling, highlighting its potential role in regulating inflammatory responses in fat cells.
Article Abstract
Objective: To determine the involvement of TNF-α and glycine receptors in the inhibition of pro-inflammatory adipokines in 3T3-L1 cells.
Methods: RT-PCR evidenced glycine receptors in 3T3-L1 adipocytes. 3T3-L1 cells were transfected with siRNA for the glycine (Glrb) and TNF1a (Tnfrsf1a) receptors and confirmed by confocal microscopy. Transfected cells were treated with glycine (10 mM). The expressions of TNF-α and IL-6 mRNA were measured by qRT-PCR, while concentrations were quantified by ELISA.
Results: Glycine decreased the expression and concentration of TNF-α and IL-6; this effect did not occur in the absence of TNF-α receptor due to siRNA. In contrast, glycine produced only slight changes in the expression of TNF-α and IL-6 in the absence of the glycine receptor due to siRNA. A docking analysis confirmed the possibility of binding glycine to the TNF-α1a receptor.
Conclusion: These findings support the idea that glycine could partially inhibit the binding of TNF-α to its receptor and provide clues about the mechanisms by which glycine inhibits the secretion of pro-inflammatory adipokines in adipocytes through the TNF-α receptor.
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| http://dx.doi.org/10.1007/s00011-021-01462-1 | DOI Listing |
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