Ribozymes with modular architecture constitute an attractive class of structural platforms for design and construction of nucleic acid nanostructures with biological functions. Through modular engineering of the Tetrahymena ribozyme, we have designed unit RNAs (L-RNAs), assembly of which formed ribozyme-based closed trimers and closed tetramers. Their catalytic activity was dependent on oligomer formation. In this study, the structural variety of L-RNA oligomers was extended by tuning their structural elements, yielding closed pentamers and closed hexamers. Their assembly properties were analyzed by electrophoretic mobility shift assay (EMSA) and atomic force microscopy (AFM).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/cbic.202100109 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Piezo-Capacitive Flexible Pressure Sensor with Magnetically Self-Assembled Microneedle Array.
ACS Sens
January 2025
CAS Key Laboratory of Magnetic Materials and Devices, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, P. R. China.
Flexible pressure sensors are pivotal in advancing artificial intelligence, the Internet of Things (IoT), and wearable technologies. While microstructuring the functional layer of these sensors effectively enhances their performance, current fabrication methods often require complex equipment and time-consuming processes. Herein, we present a novel magnetization-induced self-assembly method to develop a magnetically grown microneedle array as a dielectric layer for flexible capacitive pressure sensors.
View Article and Find Full Text PDFMolecular Engineering of Amino Acid Crystals with Enhanced Piezoelectric Performance for Biodegradable Sensors.
Angew Chem Int Ed Engl
January 2025
Nanjing University, Department of Physics, 22 Hankou Road, 210093, Nanjing, CHINA.
Amino acid crystals have emerged as promising piezoelectric materials for biodegradable and biocompatible sensors; however, their relatively low piezoelectric coefficients constrain practical applications. Here, we introduce a fluoro-substitution strategy to overcome this limitation and enhance the piezoelectric performance of amino acid crystals. Specifically, we substituted hydrogen atoms on the aromatic rings of L-tryptophan, L-phenylalanine, and N-Cbz-L-phenylalanine with fluorine, resulting in significantly elevated piezoelectric coefficients.
View Article and Find Full Text PDFProximity-activated guide RNA of CRISPR-Cas12a for programmable diagnostic detection and gene regulation.
Nucleic Acids Res
January 2025
Beijing Key Laboratory for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing 100083, China.
The flexibility and programmability of CRISPR-Cas technology have made it one of the most popular tools for biomarker diagnostics and gene regulation. Especially, the CRISPR-Cas12 system has shown exceptional clinical diagnosis and gene editing capabilities. Here, we discovered that although the top loop of the 5' handle of guide RNA can undergo central splitting, deactivating CRISPR-Cas12a, the segments can dramatically restore CRISPR function through nucleic acid self-assembly or interactions with small molecules and aptamers.
View Article and Find Full Text PDFNOD-like receptors (NLRs) are intracellular immune receptors that detect pathogen-associated cues and trigger defense mechanisms, including regulated cell death. In filamentous fungi, some NLRs mediate heterokaryon incompatibility, a self/non-self recognition process that prevents the vegetative fusion of genetically distinct individuals, reducing the risk of parasitism. The and NLRs in are highly polymorphic incompatibility genes ( genes) whose products recognize different alleles of the gene via a sensor domain composed of WD40 repeats.
View Article and Find Full Text PDFFlexible Moisture-Driven Electricity Generators Based on Heterogeneous Gels and Carbon Nanotubes.
ACS Appl Mater Interfaces
January 2025
College of Textile and Clothing Engineering, Soochow University, 199 Ren'ai Road, Suzhou 215123, China.
Recently developed asymmetric heterogeneous moisture-driven electricity generators (AHMEGs) are advantageous for harvesting energy from ubiquitous moisture due to their superior output performance and possible flexibility. However, the regeneration of AHMEG has seldom been explored. Here, we report the fabrication of flexible AHMEGs with regeneration ability simply by asymmetrically incorporating carbon nanotubes into a bilayer-structured gel with heterogeneities of both hygroscopicity and charge.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!