AI Article Synopsis
- Whole-exome sequencing (WES) was explored for genetic research in non-human primates (NHP) like olive baboons, marmosets, and vervet monkeys using human-specific reagents since NHP-specific ones are lacking.
- The study utilized the SureSelect Human All Exon V6 panel for exome capture and detected a high percentage of protein-coding genes in these primates.
- The approach proved effective and cost-efficient, revealing single nucleotide variants that may be linked to human diseases, highlighting its potential for genetic variant discovery in NHPs.
Article Abstract
Background: Whole-exome sequencing (WES) can expedite research on genetic variation in non-human primate (NHP) models of human diseases. However, NHP-specific reagents for exome capture are not available. This study reports the use of human-specific capture reagents in WES for olive baboons, marmosets, and vervet monkeys.
Methods: Exome capture was carried out using the SureSelect Human All Exon V6 panel from Agilent Technologies, followed by high-throughput sequencing. Capture of protein-coding genes and detection of single nucleotide variants were evaluated.
Results: Exome capture and sequencing results showed that more than 97% of old world and 93% of new world monkey protein coding genes were detected. Single nucleotide variants were detected across the genomes and missense variants were found in genes associated with human diseases.
Conclusions: A cost-effective approach based on commercial, human-specific reagents can be used to perform WES for the discovery of genetic variants in these NHP species.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407272 | PMC |
| http://dx.doi.org/10.1111/jmp.12524 | DOI Listing |
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