This paper provides a detailed analysis of three common NMR probe circuits (untuned, tuned, and impedance-matched) and studies their effects on multi-pulse experiments, such as those based on the Carr-Purcell-Meiboom-Gill (CPMG) pulse sequence. The magnitude of probe dynamics effects on broadband refocusing pulses are studied as a function of normalized RF bandwidth. Finally, the probe circuit models are integrated with spin dynamics simulations to design hardware-specific RF excitation and refocusing pulses for optimizing user-specified metrics such as signal-to-noise ratio (SNR) in grossly inhomogeneous fields. Preliminary experimental results on untuned probes are also presented.
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http://dx.doi.org/10.1016/j.jmr.2021.106975 | DOI Listing |
Biochim Biophys Acta Mol Cell Biol Lipids
January 2025
Department of Applied Chemistry for Environment, School of Biological and Environmental Sciences, Kwansei Gakuin University, 1 Gakuen-Uegahara, Sanda, Hyogo 669-1330, Japan.
Carotenoids are naturally occurring pigments essential for both light-harvesting and photoprotection in photosynthetic processes. Among these, carbonyl-containing carotenoids exhibit distinctive excited state properties due to the presence of intramolecular charge transfer (ICT) in their excited states. In this study, we synthesized a novel family of carotenoid analogs with varying numbers of conjugated double bonds, denoted as the Ind series, and conducted femtosecond pump-probe spectroscopy on these molecules in both acetone and n-hexane.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
January 2025
Key Laboratory for Microstructural Material Physics of Hebei Province, School of Science, Yanshan University, Qinhuangdao 066004 PR China. Electronic address:
2-{[3-(1H-benzoimidazol-2-yl)-2-hydroxy-5-methylbenzylidene] amino}-benzoic acid (HBIo) based on proton transfer can serve as the fluorescent probe for detecting heavy metal ions. The excited-state intramolecular proton transfer (ESIPT) reaction mechanism of the HBIo chromophore with an intramolecular asymmetric double hydrogen bond in different solvents are investigated. The reaction barrier of the ESIPT along hydrogen bond O1-H2···N3 is higher than that of ESIPT along O4-H5···N6, which indicates that the double ESIPT is a stepwise process.
View Article and Find Full Text PDFJ Am Chem Soc
January 2025
State Key Laboratory of Molecular Reaction Dynamics, CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Directly probing the heterogeneous conformations of intracellular proteins within their native cellular environment remains a significant challenge in mass spectrometry (MS). Here, we establish an in-cell MS and ultraviolet photodissociation (UVPD) strategy that directly ejects proteins from living cells into a mass spectrometer, followed by 193 nm UVPD for structural analysis. Applying this approach to calmodulin (CaM), we reveal that it adopts more extended conformations within living cells compared with purified samples , highlighting the unique influence of intracellular environments on protein folding.
View Article and Find Full Text PDFJ Am Chem Soc
January 2025
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, Dongchuan Road 500, Shanghai 200241, P. R. China.
Host-guest supramolecular fluorescence probes have garnered significant attention in the detection and sensing of bioactive molecules due to their functionalization potential, adjustable physical properties, and high specificity. However, such probes that reliably, rapidly, and specifically measure neurotransmitter dynamics at the cellular and in vivo level have yet to be reported. Herein, we present a supramolecular fluorescent chemosensor designed for norepinephrine (NE) detection, showing an exceptional response and specificity through host-guest complexation.
View Article and Find Full Text PDFNat Mater
January 2025
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Cells use 'active' energy-consuming motor and filament protein networks to control micrometre-scale transport and fluid flows. Biological active materials could be used in dynamically programmable devices that achieve spatial and temporal resolution that exceeds current microfluidic technologies. However, reconstituted motor-microtubule systems generate chaotic flows and cannot be directly harnessed for engineering applications.
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