AI Article Synopsis
- The study highlights the role of the calcium channel Polycystin-2 (Pkd2) in early left-right (LR) symmetry but questions if it’s the only player involved in this process.
- Pkd2 homozygous mutants exhibited disturbed flow dynamics, indicating a broader impact on LR development.
- Both whole-cell and LR organizer-specific knockdowns of Pkd2 resulted in shorter cilia and further abnormalities in flow dynamics, showing that the effects of Pkd2 reduction extend beyond just mechanosensation to influence cell shape and cilia length, ultimately affecting LR behavior.
Article Abstract
The left-right (LR) field recognizes the importance of the mechanism involving the calcium permeable channel Polycystin-2. However, whether the early LR symmetry breaking mechanism is exclusively Polycystin-2 has not been tested. For that purpose, we need to be able to isolate the effects of decreasing the levels of Pkd2 protein from any eventual effects on flow dynamics. Here we demonstrate that () homozygous mutants have abnormal flow dynamics. In addition, we performed one cell stage Pkd2 knockdowns and LR organizer specific Pkd2 knockdowns and observed that both techniques resulted in shorter cilia length and abnormal flow dynamics. We conclude that Pkd2 reduction leads to LR defects that cannot be assigned exclusively to its putative role in mediating mechanosensation because indirectly, by modifying cell shape or decreasing cilia length, Pkd2 deficit affects LR flow dynamics.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047213 | PMC |
| http://dx.doi.org/10.3389/fcell.2021.624531 | DOI Listing |
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