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Isolation and development of bovine primary respiratory cells as model to study influenza D virus infection. | LitMetric

Isolation and development of bovine primary respiratory cells as model to study influenza D virus infection.

Virology

M. H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, 40546, USA. Electronic address:

Published: July 2021

AI Article Synopsis

  • Influenza D virus (IDV) is a new virus affecting respiratory health in cattle, but studying it has been challenging due to the lack of specific cell models to mimic bovine airway cells.
  • Researchers successfully developed primary respiratory epithelial cells from different parts of a calf's respiratory system, showing typical characteristics of epithelial cells and expressing specific sialic acids necessary for virus attachment.
  • IDV was able to replicate at high levels in these bovine cells, while other influenza viruses had lower replication rates, indicating that these cells could serve as a useful model for studying IDV and related respiratory infections.

Article Abstract

Influenza D virus (IDV) is a novel type of influenza virus that infects and causes respiratory illness in bovines. Lack of host-specific in vitro model that can recapitulate morphology and physiology of in vivo airway epithelial cells has impeded the study of IDV infection. Here, we established and characterized bovine primary respiratory epithelial cells from nasal turbinate, soft palate, and trachea of the same calf. All three cell types showed characteristics peculiar of epithelial cells, polarized into apical-basolateral membrane, and formed tight junctions. Furthermore, these cells expressed both α-2,3- and α-2,6-linked sialic acids with α-2,3 linkage being more abundant. IDV strains replicated to high titers in these cells, while influenza A and B viruses exhibited moderate to low titers, with influenza C virus replication not detected. These findings suggest that bovine primary airway epithelial cells can be utilized to model infection biology and pathophysiology of IDV and other respiratory pathogens.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205979PMC
http://dx.doi.org/10.1016/j.virol.2021.04.003DOI Listing

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